Marked temperature optimum for acridine-curing of an Flac factor in Escherichia coli K-12
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چکیده
منابع مشابه
Temperature-Sensitive Mutants of Escherichia coli K-12
Crosses were carried out at 34 C and 42 C between eight pairs of isogenic strains of Escherichia coli K-12. The donor and recipient of each pair carried the same mutation for temperature-sensitive deoxyribonucleic acid (DNA) synthesis; they differed only in the presence of F-lac in the donor and a spectinomycin-resistance marker in the recipient. A different temperature-sensitive mutation was p...
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Wild-type sex factors of Escherichia coli K-12 can be cured from host cells by growth at temperatures above 42 C. This phenomenon is obscured by reinfection of cured cells if the culture is allowed to reach cell densities above 10(5)/ml. The rate of episome loss is dependent on the genetic (chromosomal) background of the host.
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SYSTEM of genetic transduction has been discovered in the sexually fertile A K-12 strain of Escherichia coli. This transduction is mediated by lambda, a temperate phage for which K-12 is normally lysogenic. The distinctive features of the lambda-K-12 system include the following: (1) The transductions are limited to a cluster of genes for galactose fermentation. The GaZ loci are closely linked ...
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Since the first reports by Cavalli-Sforza (9) and Hayes (24) of strains of Escherichia coli K-12 which are conjugational donors of chromosomal markers at high frequency (Hfr strains), many other Hfr strains have been isolated and our understanding of the mechanisms involved in Hfr formation has increased considerably. All Hfr strains arise from the integration of a conjugative plasmid into the ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1977
ISSN: 0378-1097
DOI: 10.1016/0378-1097(77)90093-3